mkn45 human gc cell lines  (Procell Inc)

Journal: Oncology Reports

Article Title: NR4A1 mediates chemotherapy-induced senescence via the PI3K/AKT pathway in gastric cancer cells

doi: 10.3892/or.2026.9080

Figure Lengend Snippet: OXA induces senescence was verified in gastric cancer cells. (A and B) SA-β-Gal staining of Ctrl and OXA groups and quantification of SA-β-Gal positive cells in AGS and MKN45 cells. (C) p21 protein expression and quantification. (D and E) Immunofluorescence of γ-H2AX and quantification. Red fluorescence indicates γ-H2AX staining, and blue fluorescence reflects nuclear staining with DAPI. (F) mRNA levels of the senescence-associated secretory phenotype factors. (G) CDK4, cyclin D1, RB, p-RB protein expression, and quantification in the two groups. Data represent the mean ± SD of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. OXA, oxaliplatin; SA-β-Gal, senescence-associated β-galactosidase; p-, phosphorylated.

Article Snippet: AGS and MKN45 human GC cell lines were acquired from the Procell Life Science & Technology Co., Ltd. and maintained in RPMI-1640 containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO 2 condition.

Techniques: Staining, Expressing, Immunofluorescence, Fluorescence

Journal: Journal of Cancer

Article Title: Anti-CD47 Antibody Enhances the Efficacy of Chemotherapy in Patients with Gastric Cancer Liver Metastasis

doi: 10.7150/jca.80725

Figure Lengend Snippet: Knockdown of CD47 inhibits GC development in mouse heterotopic xenograft model. ( A ) CD47 expression in MKN45 cell lines after transfection with sh-CD47 plasmid. ( B ) Bioluminescence images of MKN45 tumors 60 days after engraftment. ( C ) Relative luciferase intensity of MKN45 cell lines after transfection with sh-CD47 plasmid (n = 5 for each group ; ** P < 0.01, paired t- test).

Article Snippet: The human GC cell line MKN45 (CCTCC Cat# GDC0220, RRID: CVCL_0434) was purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, PRC) and cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum at 37 °C under 5% CO 2 and constant humidity.

Techniques: Knockdown, Expressing, Transfection, Plasmid Preparation, Luciferase

Journal: Journal of Cancer

Article Title: Anti-CD47 Antibody Enhances the Efficacy of Chemotherapy in Patients with Gastric Cancer Liver Metastasis

doi: 10.7150/jca.80725

Figure Lengend Snippet: CD47 knockdown promotes phagocytosis by Kupffer cells (KCs) and increases FAK phosphorylation in the CDX model. ( A ) Morphology of isolated KCs in BALB/c nude mice. ( B ) Phagocytosis by KCs after co-culture with MNK45 cells. The extent of phagocytosis of MKN45 cells by KCs was quantified (n=3 for each group, *** P < 0.001, paired t- test). ( C-E ) Western blotting confirmed the phosphorylation of FAK, PAK1, and myosin.

Article Snippet: The human GC cell line MKN45 (CCTCC Cat# GDC0220, RRID: CVCL_0434) was purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, PRC) and cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum at 37 °C under 5% CO 2 and constant humidity.

Techniques: Knockdown, Phospho-proteomics, Isolation, Co-Culture Assay, Western Blot

Journal: Journal of Cancer

Article Title: Anti-CD47 Antibody Enhances the Efficacy of Chemotherapy in Patients with Gastric Cancer Liver Metastasis

doi: 10.7150/jca.80725

Figure Lengend Snippet: Exosomes mediate phagocytosis by KCs. ( A ) Transmission electron microscopy shows the morphology of exosomes (EXOs). Size distribution of EXOs. ( B ) Flow cytometry analysis of CD9 and CD63 expression on EXOs in the sh-NC or sh-CD47 group, respectively. ( C ) Expression of CD47 and CD63 on EXOs in the sh-NC or sh-CD47 group, respectively. ( D ) Extent of phagocytosis by macrophages in each group was quantified. The phagocytic index of the sh-CD47 group was the highest, followed by that of the sh-NCMKN45 cells and sh-CD47 EXO group and sh-CD47 MKN45 cells and sh-NC EXO group (n = 3 independent experiments, ** P < 0.01, *** P < 0.001, n.s., no significance; one-way ANOVA).

Article Snippet: The human GC cell line MKN45 (CCTCC Cat# GDC0220, RRID: CVCL_0434) was purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, PRC) and cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum at 37 °C under 5% CO 2 and constant humidity.

Techniques: Transmission Assay, Electron Microscopy, Flow Cytometry, Expressing

Journal: Journal of Cancer

Article Title: Anti-CD47 Antibody Enhances the Efficacy of Chemotherapy in Patients with Gastric Cancer Liver Metastasis

doi: 10.7150/jca.80725

Figure Lengend Snippet: CD47 antibodies inhibit tumor growth in the CDX model. ( A ) MKN45 cells were implanted into the spleen of BALB/c nude mice and allowed to grow for 1 week, followed by injection of drugs (IgG and anti-CD47 antibody B6H12, tail injection; 5-Fu, intraperitoneal injection, or combination of B6H12 and 5-Fu) three times per week. ( B ) Bioluminescence images of mice at week 4. ( C ) Treatment with B6H12 antibodies + 5-Fu reduced tumor bioluminescence in the mice compared to treatment with IgG antibodies or CD47 antibodies or 5-Fu alone (n = 5 for each group; ** P < 0.01, *** P < 0.001, one way ANOVA).

Article Snippet: The human GC cell line MKN45 (CCTCC Cat# GDC0220, RRID: CVCL_0434) was purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, PRC) and cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum at 37 °C under 5% CO 2 and constant humidity.

Techniques: Injection

Journal: Iranian Biomedical Journal

Article Title: HOTAIR Induces the Downregulation of miR-200 Family Members in Gastric Cancer Cell Lines

doi: 10.52547/ibj.26.1.77

Figure Lengend Snippet: Effect of knockdown on cell viability. MTT assay was performed to evaluate the cell proliferation in si- HOTAIR -transfected AGS and MKN45 cell lines. The data show a statistically significant decrease in the growth of si- HOTAIR -transfected cells compared to siNC and MOCK groups ( **** p < 0.0001 vs. NC)

Article Snippet: Cell lines and culture conditions AGS and MKN45 human GC cell lines were prepared from the Pasteur Institute of Iran (Tehran, Iran).

Techniques: Knockdown, MTT Assay, Transfection

Journal: Iranian Biomedical Journal

Article Title: HOTAIR Induces the Downregulation of miR-200 Family Members in Gastric Cancer Cell Lines

doi: 10.52547/ibj.26.1.77

Figure Lengend Snippet: RT-qPCR indicating the downregulation of HOTAIR expression by si- HOTAIR . (A) Expression levels of HOTAIR in AGS and MKN45 cells transfected with si- HOTAIR ; (B) expression levels of miR-200c /- 200a /- 200b in AGS cells transfected with si- HOTAIR ; (C) expression levels of miR-200a /-200b/-200c in MKN45 cells transfected with si- HOTAIR . Data are expressed as mean ± SD. * p < 0.05 and *** p < 0.001

Article Snippet: Cell lines and culture conditions AGS and MKN45 human GC cell lines were prepared from the Pasteur Institute of Iran (Tehran, Iran).

Techniques: Quantitative RT-PCR, Expressing, Transfection

Journal: Iranian Biomedical Journal

Article Title: HOTAIR Induces the Downregulation of miR-200 Family Members in Gastric Cancer Cell Lines

doi: 10.52547/ibj.26.1.77

Figure Lengend Snippet: Fold change and p value of expression analysis of miR-200 family in the loss of function study of HOTAIR in different GC cell lines

Article Snippet: Cell lines and culture conditions AGS and MKN45 human GC cell lines were prepared from the Pasteur Institute of Iran (Tehran, Iran).

Techniques: Expressing

Journal: Iranian Biomedical Journal

Article Title: HOTAIR Induces the Downregulation of miR-200 Family Members in Gastric Cancer Cell Lines

doi: 10.52547/ibj.26.1.77

Figure Lengend Snippet: Correlation between the expression level of HOTAIR and miR-200 in AGS and MKN45 GC cell lines. Pearson ’ s correlation coefficient revealed the expression levels of HOTAIR and (A) miR-200a in AGS cell line ( ** p < 0.01). (B and C) miR-200a /- 200c in MKN45 cell line were negatively correlated ( * p < 0.05)

Article Snippet: Cell lines and culture conditions AGS and MKN45 human GC cell lines were prepared from the Pasteur Institute of Iran (Tehran, Iran).

Techniques: Expressing